The importance of the bacterial cell wall in uranium (VI) biosorption

The bacterial cell envelope, in particular the cell wall, is considered the main controlling factor in the biosorption of aqueous uranium (VI) by microorganisms. However, the specific roles of the cell wall, associated biomolecules, and other components of the cell envelope are not well defined. Here we report findings on the biosorption of uranium by isolated cell envelope components and associated biomolecules, with P. putida 33015 and B. subtilis 168 investigated as representative strains for the differences in Gram-negative and Gram-positive cell envelope architecture, respectively. The cell wall and cell surface membrane were isolated from intact cells using a French Pressure Cell and characterised by X-Ray Photoelectron Spectroscopy (XPS) and Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FT-IR) spectroscopy. Uranium biosorption was investigated as a function of cell envelope component and pH, comparing with intact cells and was quantified by ICP-OES.<br>Supplementary information includes characterisation of XPS spectra, uranium speciation, additional adsorption isotherms, langmuir fittings and detailed FT-IR characterisation of cell envelope components.<br><br>Data includes raw XPS and FT-IR spectra, peak identifications and uranium isotherm fittings from data collected by ICP-OES.<br>

细菌细胞包膜，尤其是细胞壁，被认为是微生物对水溶液中铀（VI）进行生物吸附的主要控制因素。然而，细胞壁、相关生物分子及细胞包膜其他成分的具体作用尚未明确界定。本研究报道了分离的细胞包膜成分及相关生物分子对铀的生物吸附研究结果，其中恶臭假单胞菌（P. putida）33015和枯草芽孢杆菌（B. subtilis）168分别作为革兰氏阴性和革兰氏阳性细菌细胞包膜结构差异的代表菌株进行研究。采用弗氏压碎器（French Pressure Cell）从完整细胞中分离细胞壁和细胞表面膜，并通过X射线光电子能谱（X-Ray Photoelectron Spectroscopy, XPS）和衰减全反射-傅里叶变换红外光谱（Attenuated Total Reflectance-Fourier Transform Infrared, ATR-FT-IR）进行表征。研究了细胞包膜成分和pH值对铀生物吸附的影响，并与完整细胞进行比较，通过电感耦合等离子体发射光谱（Inductively Coupled Plasma Optical Emission Spectrometry, ICP-OES）进行定量分析。<br>补充信息包括XPS光谱表征、铀形态分析、额外吸附等温线、Langmuir拟合以及细胞包膜成分的详细FT-IR表征。<br><br>数据包括原始XPS和FT-IR光谱、峰鉴定结果以及基于ICP-OES采集数据的铀等温线拟合结果。