Apolipoprotein E deficiency potentiates macrophage resistance to staphylococcus aureus osteomyelitis via regulating cholesterol metabolism. Apolipoprotein E deficiency potentiates macrophage resistance to staphylococcus aureus osteomyelitis via regulating cholesterol metabolism

To explore how APOE functions in the mechanism of regulation of cholesterol metabolism during S. aureus osteomyelitis, we established S. aureus osteomyelitis models in WT mice and APOE knockout mice. We then performed gene expression profiling analysis using data obtained from RNA-seq of bone marrow from mice mentioned above at day 7 after operation. Overall design: Comparative gene expression profiling analysis of RNA-seq data for WT and APOE knockout mice with or without S. aureus osteomyelitis.

为探究载脂蛋白E（APOE）在金黄色葡萄球菌（S. aureus）骨髓炎的胆固醇代谢调控机制中所发挥的功能，我们在野生型（WT）小鼠及APOE敲除小鼠中构建了金黄色葡萄球菌骨髓炎模型。随后，针对术后第7天的上述小鼠骨髓组织的RNA测序（RNA-seq）数据开展基因表达谱分析。整体实验设计：对存在或不存在金黄色葡萄球菌骨髓炎的野生型小鼠与APOE敲除小鼠的RNA测序数据进行对比基因表达谱分析。