Additional file 4: of A comprehensive fungi-specific 18S rRNA gene sequence primer toolkit suited for diverse research issues and sequencing platforms

Annealing temperatures empirically evaluated for the most promising primer pairs. Two fungal strains, one of the Basidiomycota and one of the Ascomycota, served as template DNA. Intensity of the color indicates the strength of the amplification product detected by ethidium bromide staining. Red, template DNA from Taphrina deformans; Green, template DNA from Agaricus bisporus; *, optimal annealing temperature. (XLSX 60 kb)

针对最具应用潜力的引物对所实验测定的退火温度。实验采用两株真菌作为模板DNA，一株隶属于担子菌门（Basidiomycota），另一株隶属于子囊菌门（Ascomycota）。颜色深浅可反映溴化乙锭染色检测所得扩增产物的信号强度。红色代表源自畸形外囊菌（Taphrina deformans）的模板DNA；绿色代表源自双孢蘑菇（Agaricus bisporus）的模板DNA；* 标记最优退火温度。（XLSX 60 kb）