Responses of Pelagibacterales strains to phosphate limitation. Responses of Pelagibacterales strains to phosphate limitation

Responses of Pelagibacterales strains to phosphate limitation Overall design: Batch cultures of two different strains of P. ubique (HTCC1062 and HTCC7211) were grown in a defined artificial seawater media. Biological replicates of each strain were given no phosphate amendment, and three other cultures received an excess concentration of phosphate. Cultures were harvested for microarray analyses over a time course for the purpose of observing differences in gene expression related to phosphate limitation.

远洋杆菌目（Pelagibacterales）菌株对磷酸盐限制的响应
整体设计：将两株不同的乌普萨拉远洋杆菌（Pelagibacter ubique，HTCC1062与HTCC7211）的批量培养物接种于限定性人工海水培养基中进行培养。针对每个菌株设置生物学重复实验组，不添加磷酸盐补充；另外设置三组培养物，添加过量浓度的磷酸盐。在整个时间进程中收集培养物，用于基因芯片（microarray）分析，以观测与磷酸盐限制相关的基因表达差异。