Chromosome wide analysis of parental allele specific chromatin and DNA methylation along mouse distal chr7. Mus musculus

Imprinted genes are monoallelically expressed according to parental inheritance. The maternally and paternally inherited alleles are distinguished epigenetically by DNA methylation and histone modifications. Chromosome-wide Chromatin immunoprecipitation (ChIP) and MIRA analysis of MatDup.dist7 and PatDup.dist7 MEFs provided a panoramic map of reciprocal allele-specific histone modifications and DNA methylation at imprinted genes along distal chromosome 7 and 15. Overall design: ChIP-chip and MIRA-chip was done to map histone modifications and DNA methylation along distal chr7 in the maternal allele and paternal allele in Matdup.dist7 and Patdup.dist7 MEFs, respectively, using Nimblegen tiling arrays for distal chr7.

印记基因（imprinted genes）是一类依据亲本遗传模式实现单等位基因表达的基因。母源与父源遗传的等位基因可通过DNA甲基化和组蛋白修饰在表观遗传学层面实现区分。针对MatDup.dist7与PatDup.dist7小鼠胚胎成纤维细胞（mouse embryonic fibroblasts, MEFs）开展的全染色体染色质免疫共沉淀（Chromatin immunoprecipitation, ChIP）与MIRA分析，构建了7号及15号染色体远端区域内印记基因的等位基因特异性组蛋白修饰与DNA甲基化的相互全景图谱。本数据集整体实验设计如下：分别以MatDup.dist7与PatDup.dist7 MEFs的母源等位基因、父源等位基因作为研究对象，使用针对7号染色体远端区域的Nimblegen平铺芯片，完成ChIP芯片（ChIP-chip）与MIRA芯片（MIRA-chip）实验，以定位7号染色体远端区域的组蛋白修饰与DNA甲基化水平。