KMT9 writes the H4K12me1 histone mark and controls metabolism and proliferation of castration-resistant prostate cancer cells [ChIP-seq]. KMT9 writes the H4K12me1 histone mark and controls metabolism and proliferation of castration-resistant prostate cancer cells [ChIP-seq]

KMT9 is formed by the assembly of KMT9a and KMT9b and writes the H4K12me1 mark. The objectives of this study is to identify KMT9a, KMT9b, and H4K12me1 locations by ChIP-seq in the androgen-independent PC-3M prostate cancer cells and to show that upon KMT9a knockdown the levels of H4K12me1 mark decrease. Overall design: 16 samples correponding to ChIP and input samples were used for the study

KMT9由KMT9a与KMT9b组装形成，可催化形成H4K12me1组蛋白修饰标记。本研究的目标为：在非雄激素依赖型PC-3M前列腺癌细胞中，通过染色质免疫共沉淀测序（ChIP-seq）定位KMT9a、KMT9b及H4K12me1的基因组结合位点，并验证敲低KMT9a会导致H4K12me1的修饰水平显著下降。整体实验设计：本研究共使用16份样本，涵盖染色质免疫共沉淀（ChIP）样本与输入对照样本。