Radial glia require PDGFD/PDGFRB signaling in human but not mouse neocortex

Analysis of gene expression over serial 150um sections of a single gestational week 14.5 human neocortical specimen. The hypothesis tested with this dataset was that a transcriptional signature of radial glia (neural stem cells) could be isolated via unsupervised gene coexpression analysis due to variation in the abundance of this cell type from section to section. This dataset is the first of its kind generated using this method (Gene Coexpression Analysis of Serial Sections, or GCASS). Total RNA obtained from serial sections of a single GW14.5 human neocortical specimen.

本数据集围绕单例妊娠14.5周人类新皮层标本的连续150微米厚度切片展开基因表达分析。本数据集所验证的科学假说为：鉴于不同切片间放射状胶质细胞（radial glia，神经干细胞）的丰度存在差异，可通过无监督基因共表达分析（unsupervised gene coexpression analysis）分离得到该细胞类型的转录特征。本数据集是采用连续切片基因共表达分析（Gene Coexpression Analysis of Serial Sections，缩写GCASS）方法构建的同类首个数据集。本次分析所用总RNA提取自单例GW14.5人类新皮层标本的连续切片。