Subunit vaccine elicited lung TRM cells use fibroblast IL-17R signaling to provide serotype independent immunity against hypervirulent K. pneumoniae

Subunit vaccine conferred lung protection against heterologous strain through the augmentaion of lung tissue resident memmory CD4+ T-cells. ①We immunized WT C57BL/6 mice with OmpX+LTA1 or heat killed Klebsiella pneumoniae (ATCC 43816) twice 3 weeks apart, and 1 more week later we euthanized and removed the lung for single cell suspensions in addition to naive spleen cells. ②Our data had indicated that 24 hours post pulmonary infection of K1 strain at 10e4 CFU reveals quite higher bacterial burden in Il17raDermoCre postivie mice than in Il17raDermoCre negative mice, and therefore we harvested lung tissues to obatin RNA for bulk RNAseq at 4 hours post infection because any differences in gene expression would not be due to differences in lung bacterial burdens.

亚单位疫苗（subunit vaccine）通过增强肺组织驻留记忆CD4+ T细胞，实现针对异源毒株的肺部保护。①我们以OmpX+LTA1或热灭活肺炎克雷伯菌（Klebsiella pneumoniae，ATCC 43816）免疫野生型（Wild Type，WT）C57BL/6小鼠，免疫间隔为3周，共免疫2次；免疫1周后处死小鼠，采集肺部组织制备单细胞悬液，同时采集未免疫小鼠的脾脏细胞。②我们的研究数据显示，以10^4 CFU（菌落形成单位，Colony-Forming Unit）的K1毒株经肺部感染24小时后，Il17raDermoCre阳性小鼠的肺部细菌载量显著高于Il17raDermoCre阴性小鼠；因此我们在感染后4小时采集肺部组织提取RNA，用于批量RNA测序（bulk RNAseq），因为此时基因表达的差异不会受肺部细菌载量差异的影响。