Assessing endocrine and immune parameters in human immunodeficiency virus-infected patients before and after the immune reconstitution inflammatory syndrome

ABSTRACT Objective The present study compares immune and endocrine parameters between HIV-infected patients who underwent the Immune Reconstitution Inflammatory Syndrome (IRIS-P) during antiretroviral therapy (ART) and HIV-patients who did not undergo the syndrome (non-IRIS-P). Materials and methods Blood samples were obtained from 31 HIV-infected patients (15 IRIS-P and 16 non-IRIS-P) before ART (BT) and 48 ± 2 weeks after treatment initiation (AT). Plasma Interleukin-6 (IL-6) and Interleukin-18 (IL-18) were determined by ELISA. Cortisol, dehydroepiandrosterone sulfate (DHEA-S) and thyroxin concentrations were measured using chemiluminescence immune methods. Results Concentrations of IL-6 (7.9 ± 1.9 pg/mL) and IL-18 (951.5 ± 233.0 pg/mL) were significantly higher (p 0.05). Levels of DHEA-S in IRIS-P decreased AT (1080.5 ± 124.2 vs. 782.5 ± 123.8 ng/mL, p

摘要 研究目的 本研究对比了抗逆转录病毒治疗（antiretroviral therapy, ART）期间出现免疫重建炎症综合征（Immune Reconstitution Inflammatory Syndrome, IRIS-P）的HIV感染者与未出现该综合征的HIV感染者（非IRIS-P组）的免疫与内分泌参数。材料与方法 本研究纳入31例HIV感染者（15例IRIS-P组，16例非IRIS-P组），分别在抗逆转录病毒治疗前（BT）及治疗启动后48±2周（AT）采集血液样本。采用酶联免疫吸附试验（Enzyme-Linked Immunosorbent Assay, ELISA）检测血浆白细胞介素-6（Interleukin-6, IL-6）与白细胞介素-18（Interleukin-18, IL-18）水平；采用化学发光免疫法检测皮质醇、脱氢表雄酮硫酸盐（dehydroepiandrosterone sulfate, DHEA-S）及甲状腺素浓度。结果 IRIS-P组患者血浆IL-6（7.9±1.9 pg/mL）与IL-18（951.5±233.0 pg/mL）浓度显著升高（p 0.05）。IRIS-P组患者的DHEA-S水平在治疗后出现下降（1080.5±124.2 vs. 782.5±123.8 ng/mL，p ）