VC1295 egg-5(ok1781)I | 2011-09-06T16:09:36+01:00
收藏Mendeley Data2024-01-31 更新2024-06-27 收录
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https://zenodo.org/record/1003551
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This experiment is part of the C.elegans behavioural database. For more information and the complete collection of experiments visit http://movement.openworm.org preview link : https://www.youtube.com/watch?v=3GwnDFVaHUU strain : VC1295 timestamp : 2011-09-06T16:09:36+01:00 gene : egg-5 chromosome : I allele : ok1781 strain_description : egg-5(ok1781)I sex : hermaphrodite stage : adult ventral_side : clockwise media : NGM agar low peptone arena : style : petri size : 35 orientation : away food : OP50 habituation : 30m wait who : Laura Grundy protocol : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts. lab : name : William R Schafer location : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK software : name : tierpsy (https://github.com/ver228/tierpsy-tracker) version : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219 featureID : @OMG base_name : R12E2.10 (ok1781)X on food L_2011_09_06__16_09_36___6___8 total time (s) : 899.033 frames per second : 30.03 video micrometers per pixel : 4.57571 number of segmented skeletons : 26836
本实验为秀丽隐杆线虫(Caenorhabditis elegans,C. elegans)行为数据库的组成部分。如需获取更多信息及完整实验数据集,请访问 http://movement.openworm.org。预览链接:https://www.youtube.com/watch?v=3GwnDFVaHUU。
菌株:VC1295;时间戳:2011-09-06T16:09:36+01:00;基因:egg-5;染色体:I号;等位基因:ok1781;菌株描述:egg-5(ok1781)I;性别:雌雄同体;发育阶段:成虫;腹侧朝向:顺时针;培养基:NGM低蛋白胨琼脂培养基;行为箱:35mm培养皿;摆放朝向:背离食物;饲喂菌株:大肠杆菌OP50;适应静置时长:30分钟;实验执行者:Laura Grundy;实验方案:采用E. Yemini等人发表于《Nature Methods》的实验方法,DOI:10.1038/nmeth.2560,实验开始前30分钟将线虫转移至行为箱。
实验室负责人:William R Schafer;所属单位:英国剑桥希尔斯路分子生物学MRC实验室(MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK);分析软件:Tierpsy(https://github.com/ver228/tierpsy-tracker),版本号:cbfc23eb4f1ac2f29be75ade7a937eed58a5b219;特征ID:@OMG;基础文件名:R12E2.10 (ok1781)X on food L_2011_09_06__16_09_36___6___8;总录制时长:899.03秒;录制帧率:30.03帧/秒;像素比例尺:4.57571微米/像素;分割得到的线虫骨架总数:26836。
创建时间:
2024-01-31



