Table_3_The PNA mouse may be the best animal model of polycystic ovary syndrome.csv

Polycystic ovary syndrome (PCOS) exerts negative effects on females of childbearing age. It is important to identify more suitable models for fundamental research on PCOS. We evaluated animal models from a novel perspective with the aim of helping researchers select the best model for PCOS. RNA sequencing was performed to investigate the mRNA expression profiles in the ovarian tissues of mice with dehydroepiandrosterone (DHEA) plus high-fat diet (HFD)-induced PCOS. Meanwhile, 14 datasets were obtained from the Gene Expression Omnibus (GEO), including eight studies on humans, three on rats and three on mice, and genes associated with PCOS were obtained from the PCOSKB website. We compared the consistency of each animal model and human PCOS in terms of DEGs and pathway enrichment analysis results. There were 239 DEGs shared between prenatally androgenized (PNA) mice and PCOS patients. Moreover, 1113 genes associated with PCOS from the PCOSKB website were identified among the DEGs of PNA mice. A total of 134 GO and KEGG pathways were shared between PNA mice and PCOS patients. These findings suggest that the PNA mouse model is the best animal model to simulate PCOS.

多囊卵巢综合征（Polycystic ovary syndrome, PCOS）对育龄女性具有负面影响，筛选适配的动物模型用于多囊卵巢综合征的基础研究具有重要意义。本研究从全新视角对各类动物模型展开评估，旨在助力科研人员遴选最优的多囊卵巢综合征研究模型。本研究通过RNA测序技术，探究脱氢表雄酮（dehydroepiandrosterone, DHEA）联合高脂饮食（high-fat diet, HFD）诱导的多囊卵巢综合征模型小鼠卵巢组织的mRNA表达谱；同时从基因表达综合数据库（Gene Expression Omnibus, GEO）中获取14组数据集，其中包含8项人类样本研究、3项大鼠样本研究及3项小鼠样本研究，并从PCOSKB网站获取与多囊卵巢综合征相关的基因集。本研究从差异表达基因（differentially expressed genes, DEGs）及通路富集分析结果两个维度，对比了各动物模型与人类多囊卵巢综合征的一致性：产前雄激素暴露（prenatally androgenized, PNA）小鼠与多囊卵巢综合征患者共拥有239个共同的差异表达基因；在PNA模型小鼠的差异表达基因中，共筛选出1113个来自PCOSKB网站的多囊卵巢综合征相关基因；PNA模型小鼠与多囊卵巢综合征患者共共享134条基因本体（Gene Ontology, GO）及京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes, KEGG）通路。上述研究结果表明，PNA小鼠模型是模拟多囊卵巢综合征的最优动物模型。