cMyb and vMyb in human monocytes. Homo sapiens

The transcriptional activities of c-Myb and its oncogenic variant v-Myb were compared by expressing them in primary human monocytes using recombinant adenovirus vectors. All the samples were compared to cells infected with a control adenovirus expressing only GFP. The results showed that v-Myb, which differs from c-Myb only by N- and C-terminal deletions and eleven amino acid substitutions, has a qualitatively different transcriptional activity. Keywords: repeat Overall design: 2 replicates of each type were analyzed. Replicates were performed independently, more than a week apart.

本研究借助重组腺病毒载体，在原代人单核细胞中分别表达c-Myb及其致癌变体v-Myb，以此对比二者的转录活性。所有样本均以仅表达绿色荧光蛋白（GFP）的对照腺病毒感染的细胞作为对照样本。结果显示，仅在N端、C端存在缺失且携带11个氨基酸替换的v-Myb，其转录活性与c-Myb存在质的差异。关键词：重复；实验整体设计：对每种类型的样本均进行2次生物学重复分析，各重复实验独立开展，间隔时长超过一周。