General retardation of mRNA degradation caused by speB 5’ UTR partial deletion in Streptococcus pyogenes

The speB gene of Streptococcus pyogenes has a long 5' UTR with unknown functions. Preliminary studies showed that the partial mutation of this region led to altered expression patterns of the speB gene and ropB, its upstream gene. In this study, we used microarray to examine the effect of speB 5' UTR mutation on the S. pyogenes transcriptome. The speB ORF and a large portion of the speB 5’ UTR (-1 to -527 from ATG) was replaced with an erythromycin resistant cassette gene (ermAM) to generate a ΔIG mutant. Transcriptome profiles of the wild-type (WT) and ΔIG mutant in the mid-exponential phase and early stationary phase (ST) were examined with microarray analysis.

化脓性链球菌（Streptococcus pyogenes）的speB基因携带有一段功能未知的长5'非翻译区（5' UTR）。前期研究表明，该区域的部分突变会改变speB基因及其上游基因ropB的表达模式。本研究利用微阵列（microarray）技术，探究speB 5'非翻译区突变对化脓性链球菌转录组的影响。我们将speB开放阅读框（ORF）以及speB 5'非翻译区的大部分片段（距翻译起始密码子ATG的-1至-527位）替换为红霉素抗性盒基因ermAM，成功构建ΔIG突变株。分别对中期指数生长期与早期稳定期（ST）的野生型（WT）菌株及ΔIG突变株进行转录组表达谱的微阵列分析。