Epigenetic reprogramming of intestinal stemness by dietary arachidonic acid [Organoid]. Epigenetic reprogramming of intestinal stemness by dietary arachidonic acid [Organoid]

Single cell transcriptomic profiling (sc RNA-seq) of arachidonic acid (AA) induced gene expression pogram associated with regeneration in intestinal organoids. Overall design: Single cells (from intestinal organoids treated with vehicle, arachidonic acid or 6,16-dimethyl PGE2) were flow-sorted and then loaded into 10X Chromium platform.

本数据集为花生四烯酸（arachidonic acid, AA）诱导的肠道类器官再生相关基因表达程序的单细胞转录组谱分析（sc RNA-seq）。整体实验设计：分别以溶剂对照、花生四烯酸或6,16-二甲基前列腺素E2（6,16-dimethyl PGE2）处理肠道类器官，从中分离的单细胞经流式分选后，上样至10X Chromium平台开展实验。