Genome-wide Transcriptional Analyses in Anopheles Mosquitoes Reveal an Unexpected Association Between Salivary Gland Gene Expression and Insecticide Resistance

Identification of genes associated with bendiocarb resistance. Mosquitoes collected as larvae from Nagongera and Kihihi, Uganda. Bendiocarb-resistant and unexposed female mosquitoes selected using standard WHO tube bioassays. RNA was extracted from pools of five individuals identified as An. gambiae s.s. Insecticide-susceptible mosquitoes from the Kisumu strain were included as controls. RNA hybridized in an interwoven loop design to compare four biological replicates each of resistant, unexposed, and laboratory mosquitoes.

本研究旨在鉴定与苯威（bendiocarb）抗性相关的基因。实验所用蚊虫采自乌干达纳贡杰拉（Nagongera）与基希希（Kihihi）的幼虫种群。采用世界卫生组织（WHO）标准筒式生物测定法，筛选出苯威抗性与未接触药剂的雌性蚊虫。从经鉴定为冈比亚按蚊（An. gambiae s.s.）的5只个体混合样本中提取RNA。以基苏姆（Kisumu）品系的杀虫剂敏感型蚊虫作为对照。采用交错循环设计对RNA进行杂交，分别比较抗性组、未接触组及实验室饲养组蚊虫的4次生物学重复样本。