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Absorption spectra of chromatic acclimators grown in different light colors.

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Figshare2020-10-02 更新2026-04-28 收录
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Fluorescence excitation spectra of Synechococcus A15-62, a chromatic acclimator that adjusts its PUB:PEB ratio. The spectra show excitation wavelengths absorbed by the cells and subsequently emitted by the phycobilisomes as fluorescence at 580 nm, when the cells are grown in blue light (blue line) or green light (green line). Absorption spectra of Pseudanabaena CCY9509, a chromatic acclimator that adjusts its PEB:PCB ratio. The absorption spectra are shown for cells acclimated to green light (green line), orange light (orange line), and midway during chromatic acclimation after a switch from green to orange light (black line). Spectra in were measured in this study using methods described in Sanfilippo et al. (2019) and Stomp et al. (2008)Datasets accompany Extended Data Fig 3.

聚球藻A15-62(Synechococcus A15-62)是一种可通过调整藻尿胆素(phycourobilin, PUB)与藻红胆素(phycoerythrobilin, PEB)比例实现光色适应(chromatic acclimation)的菌株,其荧光激发光谱可反映细胞所吸收的激发光波长,此时藻胆体(phycobilisomes)会在580 nm处发射荧光;该光谱分别对应细胞在蓝光(蓝色曲线)与绿光(绿色曲线)培养条件下的检测结果。假鱼腥藻Pseudanabaena CCY9509是另一种具有光色适应能力的菌株,可通过调整藻红胆素(PEB)与藻蓝胆素(phycocyanobilin, PCB)的比例适配环境光质,其吸收光谱分别对应经绿光适应的细胞(绿色曲线)、经橙光适应的细胞(橙色曲线),以及从绿光切换至橙光后处于光色适应进程中途的细胞(黑色曲线)。本研究中所测得的所有光谱均采用Sanfilippo等(2019)与Stomp等(2008)报道的实验方法完成。本数据集为扩展数据图3(Extended Data Fig 3)的配套数据集。
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