Cellular proteins identified in purified influenza virions by both gel fractionation and MudPIT LC-MS/MS analyses.
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aGel slices were numbered consecutively from the top to the bottom of an 8�C16% gel.#From a higher percentage gel.*Best sequence coverage was obtained with the deglycosylated sample.bObserved peptides include all peptides that differ either by sequence, modification or charge.cValues represent the range when the protein was found in multiple gel slices.dA Mascot score ��50 and a Protein prophet score ��0.95 are equivalent (p��For this search a Mascot score ��38 is significant (peSequence coverage is based on peptides with unique sequence.fNCBI UniGene EST profile, TPM = Transcripts per million.
a 凝胶切片从8%–16%梯度凝胶的顶部至底部依次编号。# 样本取自更高浓度的凝胶。* 去糖基化样品可实现最优的序列覆盖度。
b 观测到的肽段涵盖所有在序列、修饰或电荷上存在差异的肽段。
c 当蛋白质在多个凝胶切片中被检测到时,数值代表其取值区间。
d Mascot得分(Mascot score)≥50且Protein Prophet得分(Protein Prophet score)≥0.95时二者等效;本次检索中,Mascot得分≥38即具有统计学显著性。
e 序列覆盖度基于具有唯一序列的肽段进行计算。
f NCBI UniGene表达序列标签(EST)谱,TPM=每百万转录本数(Transcripts per million)。
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2015-12-02



