Betaine is involved in regulation of translation through alteration of N6-methyladenosine RNA methylation in liver

In order to explore the effect of betaine on NAFLD and its mechanism, we divided mice into high-fat diet (HF) and high-fat diet with betaine (HB) groups, and used N6-methyladenosine sequence (m6A-seq), RNA-seq and mRNAs bound to ribosome-nascent chain complex sequencing (RNC-seq) to evaluate the gene expression levels in the liver of each group. Examination of anti-m6A antibody in liver tissue. Total RNA and ribosome-bound RNA were extracted from the livers of high-fat diet- (HF) and High-fat diet plus Betaine (HB)-fed mice, and the polyA+ mRNA was sequenced using Illumina HiSeq X Ten sequencer. The reads were mapped to RefSeq RNA reference sequences and the expression level were quantified by rpkM calculation.

为探究甜菜碱对非酒精性脂肪性肝病（Non-Alcoholic Fatty Liver Disease，NAFLD）的作用及其潜在机制，本研究将小鼠分为高脂饮食（HF）组与高脂饮食添加甜菜碱（HB）组，并采用N6-甲基腺苷测序（m6A-seq）、RNA测序（RNA-seq）以及核糖体新生肽链复合物结合mRNA测序（RNC-seq）技术，对各组小鼠肝脏的基因表达水平进行评估，同时对肝组织中的抗m6A抗体开展检测。本研究从高脂饮食（HF）组与高脂饮食添加甜菜碱（HB）组饲喂的小鼠肝脏中提取总RNA及核糖体结合RNA，并利用Illumina HiSeq X Ten测序仪对其中的polyA+ mRNA进行测序；将测序读段比对至RefSeq RNA参考序列，通过RPKM计算对基因表达水平进行定量。