Supplementary Materials - Antitumor activity of new chemical compounds in triple negative mammary adenocarcinoma models

Aims. According to the need for the development of new anticancer agents, we have synthetized novel bioactive compounds and aimed to determine their antitumor action. <br>Materials &amp; Methods. We describe in vitro studies evaluating the effect of 35 novel chemical compounds on two triple negative murine mammary adenocarcinoma tumors. <br>Results &amp; Conclusions. Three compounds were selected because of their high antitumor activity and their low toxicity to normal cells. Their effect on tumor cells apoptosis, clonogenicity and migratory capacity, were determined. We found that the selected compounds showed inhibition of viability and clonogenic capacity, and promotion of apoptosis. They also decreased the migratory capacity of tumor cells. The results obtained suggest the likelihood of their future use as antitumor and/or antimetastatic agents

<br>SM1: Methodology and characterization of the chemical compounds SM2: Chemical structure of the compounds belonging to each familySM3: Effect of the compounds (100 µM) on 4T1 cells viability. Cells were incubated for 36 hs in complete medium with 100 µM of each compound. Viable cell number was evaluated with WST-1. Results are shown as percentage of cell viability relative to the non-treated control (100%) and expressed as mean ± SEM. Experiments were performed in triplicate.SM4: Effect of the compounds (75 µM) on 4T1 cells viability. Cells were incubated for 36 hs in complete medium with 75 µM of each compound. Viable cell number was evaluated with WST-1. Results are shown as percentage of cell viability relative to the non-treated control (100%) and expressed as mean ± SEM. Experiments were performed in triplicate. SM5: Effect of the compounds (50 and 25 µM) on 4T1 cells viability. A) Cells were incubated for 36 hs in complete medium with 50 µM of each compound; B) Cells were incubated for 36 hs in complete medium with 25 µM of each compound. Viable cell number was evaluated with WST-1. Results are shown as percentage of cell viability relative to the non-treated control (100%) and expressed as mean ± SEM. Experiments were performed in triplicate. SM6: The percent of viability of the selected compounds at 25 µM<br>

目标。基于新型抗癌药物开发的需求，我们合成了一系列新型生物活性化合物，旨在明确其抗肿瘤作用。<br>材料与方法。本研究通过体外实验评估了35种新型化合物对两种三阴性小鼠乳腺腺癌模型的作用。<br>结果与结论。三种化合物因具有高抗肿瘤活性且对正常细胞毒性较低而被筛选出来。我们测定了这些化合物对肿瘤细胞凋亡、克隆形成能力及迁移能力的影响，发现它们可抑制肿瘤细胞活力与克隆形成能力，促进凋亡，并降低其迁移能力。