CUT&RUN chromatin profiling of H3K4me3 and H3K9me3 histone marks in spermatids and spermatocytes

Spermatogenesis is a recurring differentiation process that results in the production of male gametes within the testes. During this process, spermatogonial stem cells differentiate to form spermatocytes, which undergo two rounds of meiotic division to form haploid spermatids. Throughout spermiogenesis, round spermatids elongate to form mature sperm. To profile changes in chromatin marks between spermatocytes and spermatids, we generated CUT&RUN data of H3K4me3 and H3K9me3 marks in sorted spermatocytes and spermatids.

精子发生（Spermatogenesis）是一种周期性分化过程，其结果是在睾丸内产生雄性配子。该过程中，精原干细胞分化为精母细胞，精母细胞经两轮减数分裂形成单倍体精子细胞。在整个精子形成（Spermiogenesis）阶段，圆形精子细胞逐渐伸长，最终发育为成熟精子。为了解析精母细胞与精子细胞之间染色质修饰标记的变化，我们对分选得到的精母细胞和精子细胞进行了H3K4me3与H3K9me3标记的CUT&RUN实验，并生成了相应的数据。