MYTHO: a novel regulator of skeletal muscle autophagy and integrity [3 weeks]. MYTHO: a novel regulator of skeletal muscle autophagy and integrity [3 weeks]

Short-term MYTHO knockdown (KD) mRNA expression data for the mouse muscle (n=4 per group) were obtained using Affymetrix Mouse Clariom S Assay (Affymetrix, Santa Carla, CA) according to the manufacturer’s recommendations. Overall design: Mouse (~10-wk-old) Gastroc muscles were transfected with an AAV containing a U6 promoter, a sequence coding for a sh-RNA targeted the product of the 230025D16Rik gene (the gene coding for Mytho). A control AAV containing a scramble sh-RNA sequence under the control of the U6 promoter was injected into the contralateral leg. Mice were sacrificed 3 weeks later and total RNA was extracted. 4 muscles were used for each condition.

本研究获取了小鼠肌肉组织中短期MYTHO基因敲低（knockdown, KD）后的mRNA表达谱数据，每组样本量为4（n=4 per group），实验采用Affymetrix小鼠Clariom S基因芯片检测试剂盒（Affymetrix Mouse Clariom S Assay，厂商为位于美国加利福尼亚州圣克拉拉市的Affymetrix公司），并严格遵循厂商推荐的实验流程完成。实验整体设计如下：对约10周龄小鼠的腓肠肌（Gastroc为腓肠肌Gastrocnemius的缩写）注射携带U6启动子、靶向230025D16Rik基因（该基因编码MYTHO蛋白）的短发卡RNA（short hairpin RNA, sh-RNA）编码序列的腺相关病毒（Adeno-Associated Virus, AAV）；对侧腿部则注射携带U6启动子驱动的随机乱序sh-RNA序列的对照腺相关病毒。3周后处死小鼠，提取总RNA，每个实验条件使用4份肌肉组织样本。