Birefringence optical retardations in sections of rat calcaneal tendons cultured in vitro

The dataset identifies birefringence brilliance intensities as average gray values (GA) in sections of rat calcaneal tendons cultivated under in vitro culture conditions. Calcaneal tendons from male Wistar rats were isolated, cultured on 24 mm square colorless borosilicate glass coverslips (cov), and on 35 mm ø nonpyrogenic polystyrene plastic dishes (pla) for 8 and 12 days (8Dcov, 12Dcov, 8Dpla, and 12Dpla). Tendons that were not subjected to in vitro culture were used as controls (Control). These data provide basic information about the evaluation of changes in the optical anisotropic properties of the collagen bundles and allow the statistical determination of organizational and macromolecular characteristics of these structures.

本数据集将体外培养条件下大鼠跟腱切片中的双折射亮度强度定义为平均灰度值（GA，average gray values）。分离雄性Wistar大鼠的跟腱，分别在24 mm方形无色硼硅酸盐玻璃盖玻片（cov）和35 mm直径无热原聚苯乙烯塑料培养皿（pla）上培养8天和12天，对应组别为8Dcov、12Dcov、8Dpla和12Dpla。未进行体外培养的跟腱作为对照组（Control）。这些数据为评估胶原纤维束光学各向异性特性的变化提供了基础信息，并可通过统计方法确定这些结构的组织特征和大分子特征。