DataSheet_1_Preparation, biocontrol activity and growth promotion of biofertilizer containing Streptomyces aureoverticillatus HN6.docx

Nowadays, due to the excessive dependence on chemical fertilizers and pesticides in agricultural production, many problems, such as soil hardening and soil-borne diseases, have become increasingly prominent, which seriously restrict the sustainable development of agriculture. The application of microbial fertilizer prepared by biocontrol microorganisms can not only improve soil structure and increase fertility but also have the function of controlling diseases. Streptomyces aureoverticillatus HN6 has obvious disease prevention and growth promotive effect, which can improve the rhizosphere fertility of plants and even regulate the rhizosphere microbial community of plants. Based on the comparison of frame composting and natural composting, we used the response surface method to optimize the preparation conditions of Streptomyces HN6 bacterial fertilizer. The results showed that natural composting not only produced higher composting temperatures and maintained long high temperature periods in accordance with local conditions, but was also more suitable for composting in the field according to local conditions. Therefore, the substrate’s conductivity changed more, the ash accumulation increased, and the substrate decomposed more thoroughly. Thus, this composting method is highly recommended. Additionally, Streptomyces HN6 microbial fertilizer EC20 can reduce cowpea fusarium wilt and promote cowpea growth. The number of plant leaves, plant height and fresh weight, increased significantly in the microbial fertilizer EC20. Moreover, Streptomyces HN6 fertilizer EC20 could significantly induce soil invertase, urease and catalase activities. Our study highlights the potential use of Streptomyces HN6 as a biofertilizer to improve plant productivity and biological control of plant pathogenic fungi.

如今，农业生产中过度依赖化肥与农药，导致土壤板结、土传病害等诸多问题日益凸显，严重制约了农业的可持续发展。利用生防微生物制备的微生物肥料，不仅能够改善土壤结构、提升土壤肥力，还兼具病害防控功能。金孢轮枝链霉菌HN6（Streptomyces aureoverticillatus HN6）具有显著的防病促生效果，可改善植物根际肥力，甚至调控植物根际微生物群落结构。本研究通过对比框架堆肥与自然堆肥两种方式，采用响应面法优化了金孢轮枝链霉菌HN6菌肥的制备条件。结果表明，自然堆肥不仅可产生更高的堆肥温度且能维持更长的高温持续时间，同时更贴合当地实际，更适于田间堆肥作业。因此，该堆肥方式下基质的电导率变化更为显著，灰分积累量更高，基质腐熟程度也更彻底，故该堆肥工艺极具推广价值。此外，金孢轮枝链霉菌HN6微生物肥料EC20可降低豇豆枯萎病的发病率，并促进豇豆生长。施加该菌肥EC20后，植株的叶片数、株高及鲜重均得到显著提升。同时，金孢轮枝链霉菌HN6菌肥EC20还可显著诱导土壤蔗糖酶、脲酶及过氧化氢酶活性的升高。本研究凸显了金孢轮枝链霉菌HN6作为生物肥料的应用潜力，可用于提升作物生产力及防控植物病原真菌病害。