Sub-chronic transcriptional response in adult male MutaTMMouse following oral exposure to indeno(123,cd)pyrene in lung, liver, and forestomach tissues [IP]. Mus musculus

Polycyclic aromatic hydrocarbons (PAHs) are a class of hundreds of structurally similar chemicals ubiquitously present in our environment. They are created during the incomplete combustion of organic materials, such as oil, wood, tobacco, and charbroiled meat. As such, human exposure to mixtures of PAHs can occur through consumption of PAH-containing foods and water, inhalation of polluted air, or dermal contact. Several PAHs have been classified as carcinogenic to humans or probably carcinogenic to humans by the International Agency for Research on Cancer. Indeno(123,cd)pyrenee is one such compound. In the present study, we sought to determine the dose-dependent changes in gene expression upon oral exposure to benz(a)anthracene in the lung, liver, and forestomach tissues. Adult male MutaTMMouse were exposed to three doses of indeno(123,cd)pyrene or vehicle control (olive oil) for 28 days and sacrificed three days after the final exposure. Overall design: This experiment examined the pulmonary, hepatic, and forestomach transcriptional response of male mice exposed to indeno(123,cd)pyrene for 28 days at three different doses, including D1 (12.5 mg/kg-bw/day), D2 (25 mg/kg-bw/day), and D3 (50 mg/kg-bw/day) and vehicle control (D0). Each dose group was examined 72 hours following the final exposure. Each dose group had 4-5 biological replicates. There were a total 20, 19, and 20 samples (arrays) from the lung, liver, and forestomach, respectively, included in the final analysis using a two-colour reference design.

多环芳烃（Polycyclic Aromatic Hydrocarbons, PAHs）是一类包含数百种结构相似化学物的物质，广泛分布于自然环境中。它们由石油、木材、烟草及炭烤肉类等有机物质不完全燃烧产生。正因如此，人类可通过食用含多环芳烃的食物与饮水、吸入污染空气或皮肤接触等途径暴露于多环芳烃混合污染物中。国际癌症研究机构（International Agency for Research on Cancer）已将多种多环芳烃归类为对人类致癌或很可能对人类致癌的物质，茚并(123,cd)芘（Indeno(123,cd)pyrenee）便是其中之一。
本研究旨在探究经口暴露于苯并(a)蒽（benz(a)anthracene）后，小鼠肺、肝及前胃组织中基因表达的剂量依赖性变化。实验将成年雄性Muta™小鼠（MutaTMMouse）分别暴露于三种剂量的茚并(123,cd)芘或溶剂对照（橄榄油），持续28天，并于末次暴露3天后实施安乐死。
实验设计概述：本实验针对经三种不同剂量茚并(123,cd)芘暴露28天的雄性小鼠，探究其肺、肝及前胃组织的转录组应答情况，设置的剂量组分别为D1（12.5 mg/kg体重/天）、D2（25 mg/kg体重/天）、D3（50 mg/kg体重/天）以及溶剂对照组D0。所有剂量组均于末次暴露72小时后进行采样检测，每个剂量组设置4-5个生物学重复。最终分析采用双色参考杂交设计，共纳入肺组织样本20份、肝组织样本19份及前胃组织样本20份，所有样本均为芯片样本。