Macrophage samples from the HMDP

Identify genes involved in regulation of inflammatory responses and gene-environemnt interactions, in macrophages from a set of mouse inbred strains termed the HMDP. The HMDP is a genetically diverse mapping panel comprised of classical inbred and recombinant inbred wild type mice. The RMA values of genes were used for genome wide association as described in Bennett et al Genome Research 2010. GWAS for expression in primary macrophages of mouse inbred strains. Macrophages were exposed to bacterial lipopolysaccharide (LPS), oxidized lipids (OxPAPC) or control treatment conditions.

本研究旨在鉴定源自一组被称为HMDP的小鼠近交品系的巨噬细胞中，参与炎症应答调控以及基因-环境交互作用的基因。HMDP是一个遗传多样性定位群体，由经典近交系和重组近交系野生型小鼠构成。参照Bennett等于2010年发表于《Genome Research》期刊的方法，采用基因的RMA（Robust Multi-array Average）值开展针对小鼠近交品系原代巨噬细胞基因表达的全基因组关联研究（GWAS）。实验中，巨噬细胞分别经细菌脂多糖（LPS）、氧化脂质（OxPAPC）处理，或接受对照处理。