Bench marking the assembly of viromes using Illumina, PacBio and Nanopore reads

Illumina PacBio ONT Flye Unicycler wtdbg2 (Redbean)
Sequencing of a mock community of bacteriophages with three different sequencing technologies , in triplicate.
Reads were assembled with multiple different assembly algorithms including 
Assembled contigs were compared to the known input phage genomes to calculate completeness of genomes 

All raw reads were deposisted in the ENA.
The attached file contains assembled contigs. 
Files are named in the following manner 
Library Number, Sequencing type , assembler used 
eg  lib1_PacBio_wtdbg2.fa
Library 1 sequenced using PacBio and assembed with wtdb2 

Data Update 

With advances in base calling the ONT data was re-basecalled.  

re-called ONT data is in  ONT_recalled.tar.gz and also avalible in the SRA .

对由三种不同测序技术构建的模拟噬菌体群落进行测序，实验重复三次。采用多种不同的组装算法对原始读段进行组装，并将组装得到的连续序列与已知的噬菌体基因组进行比对，以计算基因组完整性。所有原始读段已存入欧洲核序列数据库（ENA）。所附文件包含组装得到的连续序列。文件命名方式如下：库编号、测序类型、使用的组装器，例如：lib1_PacBio_wtdbg2.fa，表示库1使用PacBio测序并使用wtdbg2进行组装。数据更新：随着碱基调用技术的进步，ONT数据进行了重新碱基调用。重新调用的ONT数据存于ONT_recalled.tar.gz文件中，亦可在SRA数据库中获取。