PRMT1 and CSNK1a1 control epidermal progenitor maintanence (PRMT1 ChIP-seq data set)

Here we determine the genome-wide binding sites of PRMT1 in primary human keratinocytes. We also investigated the impact of casein kinase inhibitor D4476 on PRMT1 genomic binding. Overall design: HA-PRMT1 was expressed at near endogenous level in primary keratinocytes. Two independent HA antibodies were used for PRMT1 ChIP-seq. The CSNK1a1 inhibitor D4476 was added to keratinocyte culture to characterize the role of CSNK1a1 on controlling PRMT1 genomic binding.

本研究鉴定了原代人角质形成细胞中蛋白质精氨酸甲基转移酶1（Protein Arginine Methyltransferase 1, PRMT1）的全基因组结合位点，同时探究了酪蛋白激酶抑制剂D4476对PRMT1基因组结合的影响。整体实验设计如下：将HA-PRMT1（HA标签，Hemagglutinin tag）以接近内源水平的方式在原代角质形成细胞中表达；采用两种独立的HA抗体开展PRMT1的染色质免疫共沉淀测序（Chromatin Immunoprecipitation sequencing, ChIP-seq）实验；向角质形成细胞培养液中添加酪蛋白激酶1α1（Casein Kinase 1 alpha 1, CSNK1a1）抑制剂D4476，以阐明CSNK1a1在调控PRMT1基因组结合过程中的作用。