Characterization of the effect of deleting the Nrg-1(neuregulin-1) gene, using the tamoxifen-inducible endothelial driver line Cdh5-CreERT in E (embryonic day) 15.5 hearts. Characterization of the effect of deleting the Nrg-1(neuregulin-1) gene, using the tamoxifen-inducible endothelial driver line Cdh5-CreERT in E (embryonic day) 15.5 hearts

To investigate the role of Nrg-1 in heart ventricular chamber development, we induced a conditional Nrg-1 deletion in endothelial cells at E15.5, with tamoxifen, and determined gene expression profiles, using RNA-seq. Overall design: Gene expression profiling with RNA-seq was performed on 4 replicate samples of control (WT) and 4 replicate samples of mutant (Nrg-1flox;Cdh5-CreERT2) (1 ventricle per replicate) at E15.5

为探究神经调节蛋白-1（Neuregulin-1，Nrg-1）在心脏心室腔发育中的作用，我们于胚胎第15.5天（E15.5）通过他莫昔芬（tamoxifen）诱导内皮细胞内Nrg-1的条件性敲除，并采用RNA测序（RNA-seq）检测基因表达谱。
实验整体设计：针对E15.5时期的对照组（野生型，WT）与突变组（Nrg-1flox;Cdh5-CreERT2）各4份生物学重复样本开展RNA-seq基因表达谱分析，每份样本取自1个心室。