Genome-wide identification of transcriptional targets for ING5. Genome-wide identification of transcriptional targets for ING5

In this study, we performed chromatin immunoprecipitation-coupled massive parallel DNA sequencing (ChIP-seq) to analyze the genome-wide transcriptional profile of ING5 in hepatocytes. ChIP was performed first in HepG2 cells with antibodies against ING5. Following ChIP, ING5-associated DNAs were amplified using non-biased conditions, labeled, and sequenced with HiSeq 2500. The raw sequencing image data were examined by the Illumina analysis pipeline, aligned to the unmasked human reference genome (GRCh37, hg19) using Bowtie2, and further analyzed by MACS (Model-based Analysis for ChIP-Seq). Overall design: Examination of transcriptional targets for ING5 in HepG2 cells.

本研究采用染色质免疫共沉淀结合大规模并行DNA测序（ChIP-seq）技术，分析肝细胞中ING5的全基因组转录谱。首先在HepG2细胞中使用针对ING5的抗体开展染色质免疫共沉淀（ChIP）实验；完成ChIP后，采用无偏倚扩增条件对与ING5结合的DNA进行扩增、标记，并通过HiSeq 2500平台完成测序。原始测序图像数据经由Illumina分析流程进行处理，使用Bowtie2比对至未屏蔽的人类参考基因组（GRCh37，hg19），随后通过MACS（Model-based Analysis for ChIP-Seq）进行后续分析。实验整体设计：探究HepG2细胞中ING5的转录靶标。