iBAT polyA RNA profiles of wild type C57BL/6N-?Gm15551 mice exposed to cold.

Cold and nutrient activated brown adipose tissue (BAT) is capable of increasing systemic energy expenditure via uncoupled respiration and secretion of endocrine factors thereby protecting mice against diet-induced obesity and improving insulin response and glucose tolerance in men. Long non-coding RNAs (lncRNAs) have recently been identified as fine tuning regulators of cellular function. While certain lncRNAs have been functionally characterised in adipose tissue, their overall contribution in the activation of BAT remains elusive. We identified lncRNAs correlating to inter- scapular brown adipose tissue (iBAT) function in high fat diet (HFD) and cold stressed mice. We focused on Gm15551 which has an adipose tissue specific expression profile, is highly upregulated during adipogenesis and downregulated by ß-adrenergic activation in mature adipocytes. Albeit we performed comprehensive transcriptional and adipocyte physiology profiling in vitro and in vivo, we could not detect an effect of gain or loss of function of Gm15551. Overall design: PolyA RNA profiles of interscapular brown adipose tissue (iBAT) from 12-week old ?Gm15551 and wild type litter mate mice housed at 4°C for 24 h.

经冷暴露与营养激活的棕色脂肪组织（brown adipose tissue, BAT）可通过解偶联呼吸与内分泌因子分泌提升全身能量消耗，从而保护小鼠抵抗饮食诱导的肥胖，并改善男性的胰岛素应答与葡萄糖耐量。长链非编码RNA（long non-coding RNAs, lncRNAs）近期被证实为细胞功能的精细调控因子。尽管部分lncRNAs已在脂肪组织中完成功能表征，但其在BAT激活过程中的整体贡献仍不明确。我们在高脂饮食（high fat diet, HFD）与冷应激小鼠中鉴定出与肩胛间棕色脂肪组织（interscapular brown adipose tissue, iBAT）功能相关的lncRNAs。我们聚焦于Gm15551，该基因具有脂肪组织特异性表达谱，在脂肪生成过程中显著上调，而在成熟脂肪细胞中经β-肾上腺素能激活后表达下调。尽管我们在体外与体内完成了全面的转录组与脂肪细胞生理学谱分析，但未检测到Gm15551功能获得或功能缺失的相关效应。实验整体设计：对饲养于4℃环境24小时的12周龄ΔGm15551小鼠与野生型同窝小鼠的肩胛间棕色脂肪组织（iBAT）进行PolyA RNA谱分析。