Antibiotic-induced dysbiosis in the SCIME™ recapitulates microbial community diversity and metabolites modulation of in vivo disease - 16s rRNA seq data

Intestinal dysbiosis is a significant concern among dog owners, and the gut health of pets is an emerging research field. In this context, the Simulator of the Canine Intestinal Microbial Ecosystem (SCIMETM) was recently developed and validated with in vivo data. The current study presents a further application of thismodel by using amoxicillin and clavulanic acid to induce dysbiosis, aiming to provoke changes in microbial community and metabolite production, which are well-known markers of the disease in vivo. Following the induction of dysbiosis, prebiotic supplementation was tested to investigate the potential for microbiota recovery under different dietary conditions. The results showed that antibiotic stimulation in the SCIMETM model can reproduce significant changes in microbial communities andmetabolic activity, including a decrease inmicrobial richness, a reduction in propionic acid production, and alterations inmicrobial composition, mimicking in vivo canine dysbiosis. Additionally, changes in ammonium and butyric acid levels induced by the tested diets were observed. In conclusion, a dynamic in vitro model simulating canine antibiotic-induced dysbiosis, capable of reproducing microbial and metabolic changes observed in vivo, has been developed and is suitable for testing the e􀀀ects of nutritional changes.

肠道菌群失调是养犬群体广泛关注的重要健康问题，宠物肠道健康亦为当前新兴的研究领域。在此背景下，研究团队近期开发了犬肠道微生物生态系统模拟器（Simulator of the Canine Intestinal Microbial Ecosystem，简称SCIMETM），并通过体内实验数据完成了模型验证。本研究进一步拓展了该模型的应用场景：采用阿莫西林与克拉维酸诱导菌群失调，以引发微生物群落结构与代谢产物合成的特征性变化——此类变化均为体内疾病状态的公认生物标志物。完成菌群失调诱导后，本研究通过添加益生元补充剂，探究不同膳食条件下微生物群的恢复潜力。实验结果表明，在SCIMETM模型中施加抗生素刺激，可重现与犬类体内菌群失调一致的显著变化，包括微生物丰富度降低、丙酸产量减少以及微生物组成改变。此外，受试膳食还可诱导铵盐与丁酸水平发生特征性变化。综上，本研究开发了一种可模拟犬类抗生素诱导菌群失调的动态体外模型，该模型能够复现体内观察到的微生物与代谢变化，适用于评估膳食改变的相关影响。