Gene expression of mouse intestine treated with azoxymethane and/or St. John's wort. Mus musculus

The aim of this study was to examine the effects of dietary supplementation of St John’s wort extract on azoxymethane-induced colorectal carcinogenesis in mice. Microarray data showed atttenuation of NF-kB and ERK-mediated pathway in colon epithelium by St. Jone's wort suggesting that the anti-inflammatory effect is a possible cancer-preventing mechanism induced by St. Jone's wort. Gene expression will be compared between mice with the control vs. St. Jone's wort. Overall design: Six-condition experiment, CSN vs SSN vs CAN vs SAN vs CAT vs.SAT Biological replicates: 3CSN, 3SSN, 3CAN, 3SAN, 3CAT and 3SAT samples.

本研究旨在探究膳食补充贯叶连翘（St John’s wort）提取物对氧化偶氮甲烷诱导的小鼠结直肠癌发生的影响。基因芯片数据显示，贯叶连翘可抑制结肠上皮中核因子κB（NF-κB）与细胞外信号调节激酶（ERK）介导的信号通路，提示其抗炎作用可能是贯叶连翘发挥防癌作用的潜在机制。后续将对比对照组与贯叶连翘处理组小鼠的基因表达差异。整体实验设计：共设置6种处理条件，分别为CSN、SSN、CAN、SAN、CAT及SAT。生物学重复设置为：各处理组均设3个生物学重复样本，即CSN、SSN、CAN、SAN、CAT及SAT样本各3例。