Metatranscriptome profiling and 18S rDNA (V4) metabarcoding of salinity-induced sexualized phytoplankton of the Scheldt river (Belgium). Scheldt_metatranscriptome

A natural phytoplankton sample was collected from the freshwater zone of the tidal Scheldt river near the city of Ghent (51°00'15.6"N 3°48'19.3"E) on 20/05/2022. The sample was filtered through a plankton net with a mesh size of 10 µm to concentrate the plankton and distributed over large 150-mL culture flasks. Part of the cultures were brought to a salinity of 10 parts per thousand (ppt) in order to induce sexual reproduction of certain centric diatom species. After 24h and 48h, two salt-treated and one control samples were harvested by filtration and flash frozen in liquid nitrogen. Deep metatranscriptome sequencing of total mRNA was performed on a NovaSeq 6000 S4 platform (150bp paired-end) and a de novo transcriptome was assembled with rnaSPAdes.

2022年5月20日，研究人员在根特市（51°00'15.6"N 3°48'19.3"E）附近潮汐斯海尔德河的淡水区域采集到天然浮游植物样本。该样本经孔径10 µm的浮游生物网过滤以浓缩浮游生物，随后分装至150 mL大型培养瓶中。将部分培养体系的盐度调整至10千分比（ppt），以诱导部分中心硅藻物种的有性生殖。分别于培养24小时和48小时后，通过过滤收集2份盐处理组样本与1份对照组样本，并用液氮快速冷冻。依托NovaSeq 6000 S4测序平台（150 bp双端测序）对总mRNA进行深度宏转录组（metatranscriptome）测序，并通过rnaSPAdes软件完成从头转录组组装。