In vitro assessment of drug-induced liver steatosis based on human dermal stem cell-derived hepatic cells.. Homo sapiens

Human skin-derived precursor cells (hSKP) are a post natal stem cell population isolated from the dermis. These cells acquire hepatic characteristics upon differentiation with hepatogenic factors. Differentiated hSKP show characteristics of hepatocyte precursor cells and respond to hepatotoxic compounds in a comparable way as human hepatocyte cultures. We used microarray analyses to evaluate the modulation of gene expression due to exposure to a steatosis-inducing compound. Overall design: hSKP obtained from 3 different donors were firstly differentiated and cultivated in the presence or absence of sodium valproate (Na-VPA). Control and Na-VPA samples were collected after 24h exposure.

人皮肤来源前体细胞（human skin-derived precursor cells，简称hSKP）是一类从真皮中分离得到的产后干细胞群。该细胞群体在促肝细胞生成因子诱导分化后可获得肝细胞特性。分化后的hSKP具备肝细胞前体细胞的特征，且对肝毒性化合物的响应模式与人类肝细胞培养物高度相近。本研究采用基因芯片分析，评估了暴露于脂肪变性诱导化合物后基因表达的调控变化。

整体实验设计：从3名不同供体中获取的hSKP首先完成分化诱导，随后分别置于添加丙戊酸钠（sodium valproate，缩写Na-VPA）与未添加丙戊酸钠的培养基中培养。分别于暴露处理24小时后收集对照组与丙戊酸钠处理组样本。