Levels of Dicer, Drosha and DGCR8 mRNA are similar in depressed suicide subjects and normal controls.

Experiment 1: RT step carried out using random hexanucleotide primers. Experiment 2: RT step carried out using gene specific primers. Data were normalized using U44, and p-values were calculated using t-test, 2-tailed, unpaired. None of the comparisons were statistically significant at p = 0.05 (paired test gave similar results).

实验1：采用随机六核苷酸引物进行反转录（Reverse Transcription, RT）反应；实验2：采用基因特异性引物进行反转录反应。所有数据以U44作为内参进行标准化处理，P值通过双侧非配对t检验计算获得。在p=0.05的显著性水平下，所有比较均无统计学意义（配对t检验结果与此一致）。