DataSheet_3_A combination of plant-based compounds and extracts acts nematicidal and induces resistance against Meloidogyne incognita in tomato.xlsx

Considering the stricter European regulations for chemical pesticides (e.g. abolishment of the use of chemical soil fumigation products, such as methyl bromide), the need for more sustainable plant protection products is strongly increasing. In this research, Product X, an innovative mixture of bio-nematicidal compounds was developed and evaluated for efficacy. Product X showed a direct nematicidal effect against the root-knot nematode Meloidogyne incognita. In pot trials with tomato plants infected with M. incognita, Product X treatment lead to a significant reduction in nematode-induced gall formation. mRNA-sequencing indicated alterations in phytohormone levels and ROS-metabolism in tomato roots upon treatment with Product X, which was subsequently biochemically validated. Increased levels of abscisic acid and peroxidase activity seem to be the main factors in the response of tomato plants to Product X. Long-term administration of Product X did not yield negative effects on tomato growth or yield. In conclusion, Product X provides a new interesting mix of bio-active compounds in the combat against root-knot nematodes.

鉴于欧洲对化学农药的监管日趋严苛，例如逐步淘汰甲基溴（methyl bromide）等化学土壤熏蒸剂的使用，市场对更具可持续性的植保产品的需求正大幅攀升。本研究中，研究人员开发了一款由杀线虫生物活性化合物复配而成的创新产品X，并对其防效进行了评估。产品X对南方根结线虫（Meloidogyne incognita）展现出直接的杀线虫活性。在感染南方根结线虫的番茄盆栽试验中，施用产品X可显著减轻线虫诱导的根结形成。mRNA测序（mRNA-sequencing）结果显示，施用产品X后，番茄根系内的植物激素水平与活性氧代谢（ROS-metabolism）均发生显著改变，该结果后续通过生化实验得到了验证。脱落酸（abscisic acid）水平升高与过氧化物酶活性增强，似乎是番茄植株响应产品X的核心调控因素。长期施用产品X不会对番茄的生长与产量产生负面影响。综上，产品X作为一款兼具创新性的生物活性化合物复配制剂，可为根结线虫的防治提供全新的有效方案。