Gene expression profiling of Chinese Hamster Ovary production cell lines

The dataset set consists of 295 microarrays from 121 individual CHO cultures producing a range of biologics including monoclonal antibodies, fusion proteins and therapeutic factors; non-producing cell lines were also included. Samples were taken from a wide range of process scales and formats that varied in terms of seeding density, temperature, medium, feed medium, culture duration and product type. Cells were sampled for gene expression analysis at various stages of the culture and bioprocess-relevant characteristics including cell density, growth rate, viability, lactate, ammonium and cell specific productivity (Qp) were determined A total of 295 microarray samples were assayed. In addition 9 continous bioprocess variables were also measured.

本数据集包含来自121株独立培养的中国仓鼠卵巢（CHO）细胞的295份微阵列样本，这些细胞可表达多种生物制品，涵盖单克隆抗体、融合蛋白与治疗性因子；同时纳入了非生产性细胞系样本。样本采集自跨度广泛的工艺规模与培养模式，其接种密度、培养温度、基础培养基、补料培养基、培养时长及产物类型均存在差异。研究人员在培养的不同阶段采集细胞进行基因表达分析，并测定了多项与生物工艺相关的特征参数，包括细胞密度、生长速率、细胞活率、乳酸盐浓度、铵盐浓度以及细胞特异性生产率（Qp）。此外，还同步测定了9项连续式生物工艺参数。