Transcriptome of vitamin D3 bolus supplementation in a cohort of 25 individuals

In this study, we analyse the in-vivo modulation of the transcriptome of human PBMCs by a bolus of vitamin D3 (80,000 IU) after 24 hours. Overall design: PBMCs of 25 healthy individuals were collected before and after an in-vivo intake of a single large bolus of 80,000 IU of vitamin D3. Analysis was performed on the cohort to find plausible vitamin D target genes.

本研究旨在分析单次大剂量维生素D3（80,000 IU）干预24小时后，人体外周血单个核细胞（Peripheral Blood Mononuclear Cells, PBMCs）转录组的体内调控变化。
整体实验设计：招募25名健康志愿者，在其单次口服80,000 IU维生素D3的给药前及给药后24小时，分别采集外周血单个核细胞；本队列分析旨在筛选可信的维生素D靶基因。