Appropriate local pH value boosting the efficacy of fibroblast growth factor to promote wound repair

To explore whether the application of FGF with different isoelectric points in wounds with different pH values, GelMA hydrogels with different pH values were prepared to maintain the wounds microenvironment with the same pH values, in which aFGF and bFGF were loaded as well.interferes with the healing process to different degrees, Overall design: We then performed gene expression profiling analysis using data obtained from RNA-seq of 21 mice in 7 groups.

为探究不同等电点的成纤维细胞生长因子（Fibroblast Growth Factor, FGF）在不同pH值创面中的应用效应，本研究制备了系列不同pH值的甲基丙烯酸明胶（GelMA）水凝胶，以维持创面微环境的pH恒定，并在该水凝胶中负载酸性成纤维细胞生长因子（acidic Fibroblast Growth Factor, aFGF）与碱性成纤维细胞生长因子（basic Fibroblast Growth Factor, bFGF）。不同等电点的FGF可对创面愈合过程造成不同程度的干扰。整体实验设计：本研究随后基于7组共21只小鼠的RNA测序（RNA-seq）数据，开展了基因表达谱分析。