Gene expression profiles in the lung following intratracheal instillation of cellulose nanofibrils and multi-walled carbon nanotubes in a murine COPD model

To evaluate the transcriptional-level biological effects of manufactured nanomaterials, a whole-genome microarray analysis was performed on the lungs of a murine chronic obstructive pulmonary disease (COPD) model. Mice were intratracheally instilled with cellulose nanofibrils (CNFs) or multi-walled carbon nanotubes (MWCNTs). Mice were intratracheally instilled with CNFs, MWCNTs (80µg/mouse), or vehicle solutions (Control). Lungs were excised 28 days post-instillation for microarray analysis.

为评估人工合成纳米材料的转录水平生物学效应，研究人员对慢性阻塞性肺疾病（chronic obstructive pulmonary disease, COPD）小鼠模型的肺部开展全基因组微阵列分析。实验小鼠经气管滴注给予纤维素纳米纤维（cellulose nanofibrils, CNFs）或多壁碳纳米管（multi-walled carbon nanotubes, MWCNTs）；具体分组处理为：分别经气管滴注CNFs、剂量为80μg/只的MWCNTs，或溶剂对照（Control）。于滴注后28天摘取小鼠肺部，用于微阵列分析。