Effects of methotrexate on S3 Drosophila cells and Drosophila ovaries. Drosophila melanogaster

Comparison of transcript abundance between control (untreated) and methotrexate treated S3 Drosophila cells and ovaries disected from female flies. Keywords: Stress response Overall design: S3 cells were exposed to 0 or 5.2 X 10^-8 M MTX for 4 days then harvested. Flies were exposed to 0 or 5 ppm MTX for 5 days then ovaries were dissected. RNA was extracted from S3 cells and ovaries. 4 S3 microarrays with one dye-swap and 2 ovary microarrays swapping dyes were hybridized to spotted cDNA microarrays.

本研究对对照组（未处理组）与甲氨蝶呤（methotrexate, MTX）处理的S3果蝇细胞，以及雌性果蝇解剖获取的卵巢的转录本丰度进行了比较。
关键词：应激反应（Stress response）
实验设计：将S3细胞分别置于不含或含5.2×10^-8 M甲氨蝶呤的培养基中培养4天后收集样本；将雌性果蝇分别暴露于不含或含5 ppm甲氨蝶呤的环境中5天后，解剖分离卵巢。分别从S3细胞和卵巢中提取RNA，采用斑点cDNA微阵列（cDNA microarray）进行杂交实验：其中S3细胞组设置4张微阵列，包含1次染料互换；卵巢组设置2张微阵列，均采用染料互换方案。