DNA methylation profiling in WT-AG-haESCs, DKO-AG-haESCs and round spermatids

We generated RRBS data to analyse the DNA methylation profiling among WT-AG-haESCs, DKO-AG-haESCs and round spermatids, we found deletion of H19 and Gtl2 DMRs do not change the methylation patterns in AG-haESCs base on all detected CpG sites. We used round spermatids as control and analysed the DNA methylation profiles of all the cell lines were by RRBS.

我们构建了简化代表性亚硫酸氢盐测序（Reduced Representation Bisulfite Sequencing, RRBS）数据集，以分析野生型孤雄单倍体胚胎干细胞（WT-AG-haESCs）、双敲除孤雄单倍体胚胎干细胞（DKO-AG-haESCs）以及圆形精子细胞的DNA甲基化谱特征。基于所有检测到的CpG位点，我们发现H19与Gtl2差异甲基化区域（Differentially Methylated Regions, DMRs）的缺失并不会改变AG-haESCs的甲基化模式。本研究以圆形精子细胞作为对照，通过RRBS对所有细胞系的DNA甲基化谱进行了分析。