Table_2_Pyrosequencing analysis of bacterial community changes in dental unit waterlines after chlorogenic acid treatment.xlsx

IntroductionThe contamination of dental unit waterlines (DUWLs) poses a significant risk of cross-infection in dentistry. Although chemical disinfectants have been effective in reducing number of bacteria, they do have limitations.
MethodsThis study aimed to investigate the potential of chlorogenic acid, a natural substance with broadspectrum antibacterial properties, for treating DUWLs. Over a period of three months, we analyzed the microbial communities in 149 DUWLs samples collected from 5 dental units using high-throughput pyrophosphate sequencing.
ResultsThe results revealed that chlorogenic acid treatment had a significant impact on the microbial community profile in the DUWLs, with the most significant changes occurring within the first 15 days and stabilization observed in the last 30 days. The predominant genera detected in the samples were Bacteroides, Lactobacillus, Streptococcus, Methylobacterium, and Phreatobacter. Additionally, the relative abundance of certain beneficial bacteria, such as Alloprevotella, Roseburia, and Blautia, increased, while the presence of opportunistic pathogens like Mycobacteria significantly decreased. The functional prediction analysis using the KEGG database indicated a decrease in the pathogenicity of the bacterial community in the DUWLs following chlorogenic acid treatment.
DiscussionThis study introduces a novel approach for the prevention and treatment of infections associated with dental care.

引言：牙科手机水管（dental unit waterlines, DUWLs）污染会给牙科临床带来显著的交叉感染风险。尽管化学消毒剂可有效减少细菌数量，但仍存在局限性。
方法：本研究旨在探究具有广谱抗菌特性的天然物质——绿原酸用于牙科手机水管治理的潜力。本研究为期三个月，采用高通量焦磷酸测序（high-throughput pyrophosphate sequencing）技术，对从5台牙科设备上采集的149份牙科手机水管样本中的微生物群落进行了分析。
结果：结果显示，绿原酸处理对牙科手机水管内的微生物群落结构具有显著影响，群落变化最显著的阶段为处理后的前15天，并在最后30天趋于稳定。样本中检出的优势菌属为拟杆菌属（Bacteroides）、乳杆菌属（Lactobacillus）、链球菌属（Streptococcus）、甲基杆菌属（Methylobacterium）以及地下水杆菌属（Phreatobacter）。此外，部分有益菌如异普雷沃氏菌属（Alloprevotella）、罗斯氏菌属（Roseburia）和布劳特氏菌属（Blautia）的相对丰度有所提升，而分枝杆菌属（Mycobacteria）等条件致病菌的检出量显著降低。通过KEGG数据库进行的功能预测分析表明，经绿原酸处理后，牙科手机水管内细菌群落的致病性有所下降。
讨论：本研究为牙科诊疗相关感染的防控提供了一种全新的解决方案。