VHL reintroduction in RCC4 cells. Homo sapiens

VHL inactivation is a major component of renal cancer. In this study we re-engineered RCC4 cells to re-express VHL and performed mRNA-sequencing on triplicates to analyse gene and transcript expression changes specific to VHL inactivation.

VHL（Von Hippel-Lindau）基因失活是肾癌发生的核心分子事件之一。本研究对RCC4肾癌细胞系进行基因工程改造，使其重新表达VHL基因，并针对三组重复样本开展mRNA测序（mRNA-sequencing），以解析与VHL基因失活特异性相关的基因及转录本表达变化。