The transcription factor OsWRKY36 regulates lamina joint development and the leaf angle in rice by modulating brassinosteroid signalling. The transcription factor OsWRKY36 regulates lamina joint development and the leaf angle in rice by modulating brassinosteroid signalling

We identified a key regulator of the rice leaf angle and plant architecture, OsWRKY36, from a rice oswrky mutant library. OsWRKY36 is highly expressed in the leaf lamina joint and promotes cell growth and expansion in adaxial parenchyma cells, leading to a greater leaf angle. Overall design: Total RNA was extracted from the lamina joint tissues and total plants of 10-day-old Nip, oswrky36 mutants, and OsWRKY36-overexpresing seedlings. Sequencing was performed using Illumina Hiseq technology.

本研究从水稻oswrky突变体文库中筛选得到调控水稻叶夹角（leaf angle）与株型（plant architecture）的关键调控因子OsWRKY36。该基因在水稻叶枕（lamina joint）中高表达，可促进近轴薄壁细胞（adaxial parenchyma cells）的生长与扩张，进而增大叶夹角。
实验设计概述：分别从10日龄日本晴（Nip）、oswrky36突变体以及OsWRKY36过表达幼苗的叶枕组织与整株植株中提取总RNA，采用Illumina Hiseq测序技术（Illumina Hiseq）进行测序。