Identification of genes important during atrial myocardial morphogenesis

Atrial cardiomyocytes undergo complex cellular behaviours after 72 hours post fertilization to build muscle structures. We aimed to identify the genes involved during this process, and therefore we seqeunced the RNA from 48 and 72 hpf atrial cardiomyocytes to uncover changes in the transcirptome that might regulate or trigger atrial morphogenesis after 72 hpf. Overall design: Comparative gene expression profiling analysis of RNA-seq data for 48 and 72 hours post fertilization wild-type zebrafish atrial cardiomyocytes.

心房心肌细胞在受精后72小时（hours post fertilization，以下简称hpf）会发生复杂的细胞行为，进而构建肌肉组织结构。本研究旨在鉴定该过程中参与调控的基因，因此对受精后48 hpf与72 hpf的心房心肌细胞的RNA进行了测序，以揭示可能调控或触发72 hpf后心房形态发生的转录组变化。整体实验设计：针对野生型斑马鱼受精后48小时及72小时的心房心肌细胞的RNA测序数据，开展比较基因表达谱分析。