Comparison of Bacillus subtilis wild type and cshA mutant at exponential versus stationary phase

Comparison of Bacillus subtilis wild type and cshA mutant at exponential versus stationary phase. Detailed description (other than provided below) of growth conditions, RNA preparation, cDNA synthesis and hybridization conditions can be found in the submitted paper. Bacillus subtilis 168 wild type and its cshA derivative strain were grown in CSE-Glu medium and cells were harvested at mid exponential (OD600 ~0.8) and early stationary phase (OD600 ~2.2-2.4). For both time points, 4 biological replicates were used and 2 were mixed after cDNA labeling, resulting 2 slides for both exponential and stationary phase. Dye swaps are included in both experiments.

枯草芽孢杆菌（Bacillus subtilis）野生型与cshA突变体在指数生长期与稳定期的比较分析。有关培养条件、RNA制备、cDNA合成及杂交条件的详细说明（除下文所述内容外），可参见已投稿的研究论文。本实验以枯草芽孢杆菌168野生型菌株及其cshA衍生突变株为研究对象，在CSE-Glu培养基中培养，分别于指数中期（OD₆₀₀≈0.8）与早期稳定期（OD₆₀₀≈2.2~2.4）收集菌体。两个时间点均设置4次生物学重复，在完成cDNA标记后将2份重复样本混合，最终指数生长期与稳定期样本各对应2张基因芯片玻片。两组实验均采用染料互换（dye swaps）实验设计。