Methodoligies for identifying lead toxicity

Quantifying impact of lead on cytokine production and gene expression in PBMCs Cells were treated with 10mkM lead acetate for one day, washed and grown in RPMI for the second day. Lead acetate was not added to matching control cells.

量化铅对人外周血单个核细胞（PBMCs）细胞因子产生及基因表达的影响。将细胞以10mkM乙酸铅处理1天，洗涤后于RPMI培养基中培养次日；设置匹配对照组细胞，不添加乙酸铅。