Expression data measured by microarray of CD4+ T cells from healthy individuals stimulated with anti-CD3/CD28. Homo sapiens

This SubSeries in the ImmVar project investigates the response of selected genes in T cells from healthy human individuals to ascertain the impact of genetic or non-genetic variation on T cell activation parameters. We measured gene expression from resting and activated CD4+ T cells derived from the peripheral blood of healthy individuals. We activated the primary T cells with anti-CD3/CD28 beads alone or with IFNb or Th17 polarizing cytokines. Overall design: We collected peripheral blood from each human donor. We isolated peripheral blood mononuclear cells by Ficoll, and negatively selected for CD4+ T cells using RosettaSep. We then either left cells unstimulated or stimulated them with beads conjugated with anti-CD3 and anti-CD28. Cells from 15 individuals were harvested at up to 5 conditions, lysed and RNA isolated to be profiled on microarray.

ImmVar项目的本次子系列数据集旨在探究健康人类个体T细胞中选定基因的表达响应，以明确遗传或非遗传变异对T细胞活化参数的影响。本研究对源自健康个体外周血的静息态与活化态CD4+ T细胞（CD4+ T cells）进行了基因表达量检测。我们通过三种方式活化原代T细胞：单独使用抗CD3/CD28磁珠，或联合IFN-β（IFNb），或联合Th17（辅助性T细胞17）极化细胞因子。

实验整体设计如下：我们从每名人类受试者处采集外周血；采用Ficoll密度梯度离心法分离外周血单个核细胞，再通过RosettaSep磁珠阴性分选获取CD4+ T细胞；随后将细胞分为未刺激组，以及经抗CD3/抗CD28偶联磁珠刺激的各组。我们从15名受试者处采集了最多5种培养条件下的细胞，经裂解、提取RNA后，通过微阵列芯片（microarray）进行转录组分析。