Bioinformatic Pipeline Scripts Amplicon Sequencing - Grey Box Grassy Woodlands

Bioinformatic scripts for the paper titled "Habitat fragmentation shifts soil microbial composition but not richness". Scripts outline the bioinformatic pipeline using DADA2 and QIIME2 to create amplicon sequence variant tables from .fastq files.The .fastq files and sequence metadata are available on the Sequence Read Archive under project number: PRJNA1298480

本套生物信息学脚本配套于题为《生境破碎化改变土壤微生物群落组成而非其丰富度》的研究论文。该脚本详述了基于DADA2与QIIME2的生物信息学分析流程，可从FASTQ格式文件中生成扩增子序列变异（amplicon sequence variant, ASV）表。相关FASTQ格式文件及序列元数据可在序列读取档案（Sequence Read Archive, SRA）数据库中获取，对应项目编号为PRJNA1298480。